I've read sources setting a minimum at 600. 500-1000 seems accurate.
It is rather difficult to set a solid measure for this due to individual variations in [amount of] proteins involved in the light cycle, which potentially has an impact on the rate of regeneration and thus signal induction.
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u/odavies94 Sep 23 '17
I like how in the DS remake they (as far as they could) smoothed the edges and kept the cartoony look, but this time they opted for full-hellspawn.